Clonejet Pcr Cloning Kit Manual
Gel Extraction Kit, Qiagen), cloning with the CloneJET PCR Cloning Kit (Thermo Scientific). (Thermo Scientific) according to the user manual. CloneEZ ® PCR Cloning Kit Cat. L00339 Technical Manual No. 0279 Update Date: 11252010 I Description. CloneJET™ PCR Cloning Kit - Thermo Scientific.
Discover our column-based GeneJET kits, and magnetic bead-based MagJET kits for high-throughput automated or manual purification. (PDF 135KB) (PDF 217KB) Thermo Scientific™ GeneJET™ Kits Product Description Thermo Scientific™ GeneJET™ Plasmid Miniprep Kit Cat. 25 preps Thermo Scientific™ GeneJET™ Plasmid Miniprep Kit Cat. 100 preps Thermo Scientific™ GeneJET™ Plasmid Midiprep Kit Cat. 25 preps Thermo Scientific™ GeneJET™ Plasmid Midiprep Kit Cat. 100 preps Thermo Scientific™ GeneJET™ Plasmid Maxiprep Kit Cat.
10 preps Thermo Scientific™ GeneJET™ Plasmid Maxiprep Kit Cat. 25 preps Thermo Scientific™ GeneJET™ Genomic DNA Purification Kit Cat. 50 preps Thermo Scientific™ GeneJET™ Genomic DNA Purification Kit Cat. 250 preps Thermo Scientific™ GeneJET™ RNA Purification Kit Cat. 50 preps Thermo Scientific™ GeneJET™ RNA Purification Kit Cat. 250 preps Thermo Scientific™ GeneJET™ PCR Purification Kit Cat. 50 preps Thermo Scientific™ GeneJET™ PCR Purification Kit Cat.
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250 preps Thermo Scientific™ GeneJET™ Gel Extraction Kit Cat. 50 preps Thermo Scientific™ GeneJET™ Gel Extraction Kit Cat.
250 preps + More Molecular Cloning Molecular cloning produces identical, recombinant DNA molecules for downstream applications, such as gene expression, protein purification, and mutational analysis. Genes of interest are cloned into plasmid vectors, which are transformed into bacterial cells to produce bacterial clones. Thermo Scientific offers the most reliable genetic content, superior enzymes and advanced PCR tools to support and streamline your entire cloning workflow. Cloning Product Description Thermo Scientific™ Rapid DNA Ligation Kit Cat. 150 reactions Thermo Scientific™ CloneJET™ PCR Cloning Kit Cat. 20 reactions Thermo Scientific™ CloneJET™ PCR Cloning Kit Cat.
40 reactions Thermo Scientific™ GeneJET™ Plasmid Miniprep Kit Cat. 50 preps Thermo Scientific™ GeneJET™ Plasmid Miniprep Kit Cat. 250 preps Thermo Scientific™ T4 DNA Ligase Cat. 200U Thermo Scientific™ T4 DNA Ligase Cat.
1000U Thermo Scientific™ T4 Polynucleotide Kinase Cat. 500U Thermo Scientific™ T4 Polynucleotide Kinase Cat. 2500U + More PCR/qPCR PCR is used to detect the presence or absence of specific DNA sequences, amplify large amounts of DNA for downstream use, or mutagenize coding DNA, for further genetic manipulation, mutational analysis, sequencing, genotyping, or cloning.Depending on the polymerase used, amplification can be highly robust, quick, or error proof, as in the case of high-fidelity enzymes. If starting material is a concern, direct PCR can be used to eliminate sample preparation altogether, or Reverse Transcription-PCR can be used if starting with mRNA. Quantitative real time PCR (qPCR) allows for real-time results with less variability due to sensitive fluorescent chemistry and elimination of post-PCR detection procedures. PCR Master Mix Product Description Thermo Scientific™ Phusion™ Flash High-Fidelity PCR Master Mix Cat. 500 reactions; Volume: 20µL Thermo Scientific™ Phusion™ Flash High-Fidelity PCR Master Mix Cat.
100 reactions; Volume: 20µL DNA Polymerase Product Description Thermo Scientific™ Phusion™ Hot Start II High-Fidelity DNA Polymerase Cat. 100U (2U/µL) Thermo Scientific™ Phusion™ Hot Start II High-Fidelity DNA Polymerase Cat. 500U (2U/µL) Thermo Scientific™ Phusion™ Hot Start II High-Fidelity DNA Polymerase Cat. 100U (2U/µL) Thermo Scientific™ Phusion™ Hot Start II High-Fidelity DNA Polymerase Cat. 500U (2U/µL) Thermo Scientific™ Phire™ Hot Start II DNA Polymerase Cat. 200 reactions; Volume: 50µL Thermo Scientific™ Phire™ Hot Start II DNA Polymerase Cat. 1000 reactions; Volume: 50µL Thermo Scientific™ Phire™ Hot Start II DNA Polymerase Cat.
Pcr Cloning Kit
Phire Green Hot Start; 200 reactions; Volume: 50µL Thermo Scientific™ Phire™ Hot Start II DNA Polymerase Cat. Phire Green Hot Start; 1000 reactions; Volume: 50µL vol qPCR Product Description Thermo Scientific™ Fermentas™ Maxima™ SYBR™ Green/ROX qPCR Master Mix (2X) Cat. 2 reactions; Volume: 1.25mL Thermo Scientific™ Fermentas™ Maxima™ SYBR™ Green/ROX qPCR Master Mix (2X) Cat.
10 reactions; Volume: 1.25mL Thermo Scientific™ Fermentas™ Maxima™ SYBR™ Green/ROX qPCR Master Mix (2X) Cat. 4 reactions; Volume: 12.5mL Thermo Scientific™ Luminaris™ Color HiGreen qPCR Master Mixes and Luminaris™ HiGreen qPCR Master Mixes (colorless) Cat.
HiGreen High ROX Thermo Scientific™ Luminaris™ Color HiGreen qPCR Master Mixes and Luminaris™ HiGreen qPCR Master Mixes (colorless) Cat. HiGreen Low ROX Thermo Scientific™ Luminaris™ Color HiGreen qPCR Master Mixes and Luminaris™ HiGreen qPCR Master Mixes (colorless) Cat. HiGreen Fluorescein Thermo Scientific™ Luminaris™ Color HiGreen qPCR Master Mixes and Luminaris™ HiGreen qPCR Master Mixes (colorless) Cat. HiGreen RT-PCR Product Description Thermo Scientific™ RevertAid™ H Minus Reverse Transcriptase Cat. 50 reactions; Volume: 20µL Thermo Scientific™ RevertAid™ Reverse Transcriptase Cat.
250 reactions; Volume: 20µL Thermo Scientific™ RevertAid™ H Minus First Strand cDNA Synthesis Kit Cat. 100 reactions Thermo Scientific™ RevertAid™ First Strand cDNA Synthesis Kit Cat.
For 100 reactions Thermo Scientific™ RiboLock RNase Inhibitor Cat. 2500U + More.